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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00051426-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00051426-M01, RRID:AB_509073
- Product name
- POLK monoclonal antibody (M01), clone 6F2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant POLK.
- Antigen sequence
MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEK
INKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQ
KAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVH
IDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSN
YHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYR
AVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEE
RQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHE
RSISPLLFEESPSDVQPPGDPFQVNFEEQNNPQIL
QNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAP
NTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLP
IRKVSGIGKVTEKMLKALGIITCTELYQQRALLSL
LFSETSWHYFLHISLGLGSTHLTRDGERKSMSVER
TFSEINKAEEQYSLCQELCSELAQDLQKERLKVLY
FDMVSLVFKFFNSKMLP- Isotype
- IgG
- Antibody clone number
- 6F2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Both high-fidelity replicative and low-fidelity Y-family polymerases are involved in DNA rereplication.
Sekimoto T, Oda T, Kurashima K, Hanaoka F, Yamashita T
Molecular and cellular biology 2015 Feb;35(4):699-715
Molecular and cellular biology 2015 Feb;35(4):699-715
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- POLK monoclonal antibody (M01), clone 6F2 Western Blot analysis of POLK expression in Hela S3 NE ( Cat # L013V3 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody (M01), clone 6F2.Lane 1: POLK transfected lysate(54.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged POLK is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
