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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AM31124PU-N - Provider product page
- Provider
- OriGene
- Product name
- POLK mouse monoclonal antibody, clone 6F2, Purified
- Antibody type
- Monoclonal
- Description
- POLK mouse monoclonal antibody, clone 6F2, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- POLK
- Isotype
- IgG
- Antibody clone number
- 6F2
- Vial size
- 50 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of POLK expression in HeLa nuclear extracts by POLK monoclonal antibody, clone 6F2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody, clone 6F2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody, clone 6F2. GAPDH (36.1 kDa) used as control.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged POLK is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Validation comment
- IHC
