600-101-295

antibody from Rockland Immunochemicals, Inc.

Targeting: POLK DINB1, DINP, POLQ

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-101-295

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-101-295, RRID:AB_2165840

Product name

Anti-POLK (DNA polymerase kappa) (GOAT) Antibody - 600-101-295

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Affinity Purified anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead).  Approximately 35 µg of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.   After blocking the membrane was probed with the primary antibody diluted to 1:1,000.  Reaction occurred overnight at 4° followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu (605-432-013) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.

Validation comment

Western Blot