- 37-3000 - Invitrogen Antibodies RPL11 antibody

Submitted references Adaptive translational reprogramming of metabolism limits the response to targeted therapy in BRAF(V600) melanoma.

Smith LK, Parmenter T, Kleinschmidt M, Kusnadi EP, Kang J, Martin CA, Lau P, Patel R, Lorent J, Papadopoli D, Trigos A, Ward T, Rao AD, Lelliott EJ, Sheppard KE, Goode D, Hicks RJ, Tiganis T, Simpson KJ, Larsson O, Blythe B, Cullinane C, Wickramasinghe VO, Pearson RB, McArthur GA
Nature communications 2022 Mar 1;13(1):1100

The exon-junction complex helicase eIF4A3 controls cell fate via coordinated regulation of ribosome biogenesis and translational output.

Kanellis DC, Espinoza JA, Zisi A, Sakkas E, Bartkova J, Katsori AM, Boström J, Dyrskjøt L, Broholm H, Altun M, Elsässer SJ, Lindström MS, Bartek J
Science advances 2021 Aug;7(32)

Ribosomal Protein L15 is involved in Colon Carcinogenesis.

Dong Z, Jiang H, Liang S, Wang Y, Jiang W, Zhu C
International journal of medical sciences 2019;16(8):1132-1141

Direct relationship between the level of p53 stabilization induced by rRNA synthesis-inhibiting drugs and the cell ribosome biogenesis rate.

Scala F, Brighenti E, Govoni M, Imbrogno E, Fornari F, Treré D, Montanaro L, Derenzini M
Oncogene 2016 Feb 25;35(8):977-89

Acrolein preferentially damages nucleolus eliciting ribosomal stress and apoptosis in human cancer cells.

Wang HT, Chen TY, Weng CW, Yang CH, Tang MS
Oncotarget 2016 Dec 6;7(49):80450-80464

mTOR inhibitors blunt the p53 response to nucleolar stress by regulating RPL11 and MDM2 levels.

Goudarzi KM, Nistér M, Lindström MS
Cancer biology & therapy 2014;15(11):1499-514

Growth inhibitory effects of large subunit ribosomal proteins in melanoma.

Kardos GR, Dai MS, Robertson GP
Pigment cell & melanoma research 2014 Sep;27(5):801-12

p53 is positively regulated by miR-542-3p.

Wang Y, Huang JW, Castella M, Huntsman DG, Taniguchi T
Cancer research 2014 Jun 15;74(12):3218-27

5S ribosomal RNA is an essential component of a nascent ribosomal precursor complex that regulates the Hdm2-p53 checkpoint.

Donati G, Peddigari S, Mercer CA, Thomas G
Cell reports 2013 Jul 11;4(1):87-98

Ribosomal protein S6 interacts with the latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus.

Chen W, Dittmer DP
Journal of virology 2011 Sep;85(18):9495-505

Ribosomal 18 S RNA processing by the IGF-I-responsive WDR3 protein is integrated with p53 function in cancer cell proliferation.

McMahon M, Ayllón V, Panov KI, O'Connor R
The Journal of biological chemistry 2010 Jun 11;285(24):18309-18

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of 293T cell lysates using Ms anti-L11 (Product # 37-3000).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of SK-OV-3 (Lane 1), Jurkat (Lane 2), HeLa (Lane 3), COS-7 (Lane 4), HEL 92.1.7 (Lane 5) and HEK-293 (Lane 6).The blots were probed with RPL11 Mouse monoclonal Antibody (Product # 37-3000, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution).A 20 kDa band corresponding to RPL11 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of L11 was performed using 70% confluent log phase U-2 OS cells treated with doxorubicin (2 µg/mL) for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RPL11 (3A4A7) Mouse Monoclonal Antibody (Product # 37-3000) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Characterization of ribosomal protein RPL15. (A) Immunofluorescence analysis of HeLa cells with rabbit alpha-RPL15 and mouse alpha-Bip, anti-alpha-tubulin, alpha-nucleolin, alpha-fibrillarin or alpha-UBF. DNA was visualized by DAPI staining. R values were obtained as described (see Materials and Methods). Scale bars, 10 mum. (B) Immunofluorescence analysis of HeLa cells with rabbit alpha-RPL15 and mouse alpha-RPL11 or mouse alpha-RPS6 and DAPI. Scale bars, 10 mum. R values were obtained as described in (A) . (C) Nuclear or cytoplasmic lysates from HeLa cells were extracted and subjected to immunoblotting by indicated antibodies. The density of each protein in each component was quantitated against the level of WCL (1/5 of total). WCL: whole cell lysate. (D) Histograms represented average proportion of RPL15 or RPL11 in Nuclear or cytoplasmic component relative to WCL in (C). All experiments were performed in triplicate. Data are shown as mean +- standard deviations.

37-3000

Antibody data