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LearnMA5-26901
antibody from Invitrogen Antibodies
Targeting: RPL10
DXS648, DXS648E, FLJ23544, L10, NOV, QM
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- MA5-26901 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL10 Monoclonal Antibody (OTI6B11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI6B11
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Chromosome clustering by Ki-67 excludes cytoplasm during nuclear assembly.
Cuylen-Haering S, Petrovic M, Hernandez-Armendariz A, Schneider MWG, Samwer M, Blaukopf C, Holt LJ, Gerlich DW
Nature 2020 Nov;587(7833):285-290
Nature 2020 Nov;587(7833):285-290
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human colon tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a RPL10 monoclonal antibody (Product # MA5-26901) at a dilution of 1:150.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a RPL10 monoclonal antibody (Product # MA5-26901) at a dilution of 1:150.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human ovary tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a RPL10 monoclonal antibody (Product # MA5-26901) at a dilution of 1:150.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a RPL10 monoclonal antibody (Product # MA5-26901) at a dilution of 1:150.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human lymphoma tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a RPL10 monoclonal antibody (Product # MA5-26901) at a dilution of 1:150.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Extended Data Figure 1. Characterization of GEM and L10 expressing stable cell lines a , Viability of HeLa cell lines stably expressing markers as indicated, measured by counting Hoechst-stained cell nuclei that were negative for the cell death marker TO-PRO-3. Cell numbers: n = 754 (wild-type), n = 930 (GEMs), n = 603 (L10-EGFP). b , Proliferation of HeLa cell lines stably expressing markers as indicated, quantified as fold-change of live cells between 0 and 24 h in time-lapse microscopy videos. Cell number at 24 h: n = 2001 (wild-type), n = 2178 (L10-EGFP), n = 2283 (GEMs). c , Mitotic duration in HeLa cell lines stably expressing markers as indicated, scored by manual measurement of the time from prometaphase to anaphase onset. Cell numbers: n = 320 (wild-type), n = 320 (GEMs), n = 320 (L10-EGFP). d, e , Validation of L10-EGFP incorporation into ribosomes. d , Cell lysate fractions separated on a sucrose gradient. Dashed lines indicate fractions loaded on the gel blotted in ( e ). e , Immunoblot analysis of ribosomal protein L10 in cell lysate fractions, isolated from cells stably expressing L10-EGFP. Annotations of endogenous L10 and L10-EGFP are based on predicted molecular weights. For gel source data, see Supplementary Fig. 1 . Bars indicate mean and error bars represent +- SD.
