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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-89335 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPLP0 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNVGSKQM QQIRMSLRGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFVF TKEDLTEIRD MLLANKVPAA ARAGAIAPCE VTVPAQNTGL GPEKTSFFQA LGITTKISRG TIEILSDVQL IKTGDKVGAS EATLLNMLNI SPFSFGLVIQ QVFDNGSIYN PEVLDITEET LHSRFLEGVR NVASVCLQIG YPTVASVPHS IINGYKRVLA LSVETDYTFP LAEKVKAFLA DPSAFVAAAP VAAATTAAPA AAAAPAKVEA KEESEESDED MGFGLFD; Positive Samples: SW620, OVCAR3, Mouse liver, Mouse thymus; Cellular Location: Cytoplasm, Nucleus
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.38 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references A Proteomic Study Suggests Stress Granules as New Potential Actors in Radiation-Induced Bystander Effects.
Tudor M, Gilbert A, Lepleux C, Temelie M, Hem S, Armengaud J, Brotin E, Haghdoost S, Savu D, Chevalier F
International journal of molecular sciences 2021 Jul 26;22(15)
International journal of molecular sciences 2021 Jul 26;22(15)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using RPLP0 Polyclonal antibody (Product # PA5-89335) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RPLP0 in extracts of various cell lines using RPLP0 Polyclonal Antibody (Product # PA5-89335) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RPLP0 was performed in HeLa cells using RPLP0 Polyclonal Antibody (Product # PA5-89335).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RPLP0 in paraffin-embedded human prostate using RPLP0 Polyclonal Antibody (Product # PA5-89335) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RPLP0 in paraffin-embedded human esophagus using RPLP0 Polyclonal Antibody (Product # PA5-89335) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RPLP0 in paraffin-embedded mouse lung using RPLP0 Polyclonal Antibody (Product # PA5-89335) at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Western blotting analysis of cyclophilin A, thioredoxin, alpha-enolase, RPLP0, HSC70, HSPA9, and CCT3 in a whole-cell extracts from T/C-28A2 bystander cells receiving the conditioned medium of low-dose irradiated chondrosarcoma cells (0.1 Gy) or non-irradiated chondrosarcoma cells (CTR).
