11290-2-AP

antibody from Invitrogen Antibodies

Targeting: RPLP0 L10E, LP0, P0, PRLP0, RPP0

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11290-2-AP

Provider

Invitrogen Antibodies

Product name

RPLP0 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPREDRATW KSNYFLKIIQ LLDDYPKCFI VGADNVGSKQ MQQIRMSLRG KAVVLMGKNT MMRKAIRGHL ENNPALEKLL PHIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ LIKTGDKVGA SEATLLNMLN ISPFSFGLVI QQVFDNGSIY NPEVLDITEE TLHSRFLEGV RNVASVCLQI GYPTVASVPH SIINGYKRVL ALSVETDYTF PLAEKVKAFL ADPSAFVAAA PVAAATTAAP AAAAAPAKVE AKEESEESDE DMGFGLFD (1-317 aa encoded by BC009867)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11290-2-AP (RPLP0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat liver tissue were subjected to SDS PAGE followed by western blot with 11290-2-AP (RPLP0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11290-2-AP (RPLP0 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat liver tissue were subjected to SDS PAGE followed by western blot with 11290-2-AP (RPLP0 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat liver tissue were subjected to SDS PAGE followed by western blot with 11290-2-AP (RPLP0 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 11290-2-AP (RPLP0 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 11290-2-AP (RPLP0 antibody) at dilution of 1:100 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RPLP0 (IP:11290-2-AP, 4ug; Detection:11290-2-AP 1:500) with HeLa cells lysate 2680 ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RPLP0 (IP:11290-2-AP, 4 ug; Detection:11290-2-AP 1:500) with HeLa cells lysate 3200 ug.