GTX104302

antibody from GeneTex

Targeting: PLK1 PLK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX104302

Provider

GeneTex

Proper citation

GeneTex Cat#GTX104302, RRID:AB_1951284

Product name

PLK1 antibody [N2C2], Internal

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

SMC5/6 is required for the formation of segregation-competent bivalent chromosomes during meiosis I in mouse oocytes.

Hwang G, Sun F, O'Brien M, Eppig JJ, Handel MA, Jordan PW
Development (Cambridge, England) 2017 May 1;144(9):1648-1660

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N-Hydroxycinnamide derivatives of osthole presenting genotoxicity and cytotoxicity against human colon adenocarcinoma cells in vitro and in vivo.

Liu LY, Huang WJ, Lin RJ, Lin SY, Liang YC
Chemical research in toxicology 2013 Nov 18;26(11):1683-91

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample(30 ?g whole cell lysate)A:Raji (GTX27908)7.5% SDS PAGEGTX104302 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PLK1 antibody [N2C2], Internal detects PLK1 protein at midbody by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformadehyde at RT for 30 min.Green: PLK1 protein stained by PLK1 antibody [N2C2], Internal (GTX104302) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human skin, using PLK1(GTX104302) antibody(10 £gg/ml).