Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PAB10069 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10069, RRID:AB_1711344
- Product name
- PLK1 (phospho T210) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of PLK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Polo-like kinase-1 controls recovery from a G2 DNA damage-induced arrest in mammalian cells.
Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/Cdc20 activation and initiation of cytokinesis.
Polo-like kinase 1 is overexpressed in prostate cancer and linked to higher tumor grades.
van Vugt MA, BrĂ¡s A, Medema RH
Molecular cell 2004 Sep 10;15(5):799-811
Molecular cell 2004 Sep 10;15(5):799-811
Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/Cdc20 activation and initiation of cytokinesis.
van Vugt MA, van de Weerdt BC, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RM, Medema RH
The Journal of biological chemistry 2004 Aug 27;279(35):36841-54
The Journal of biological chemistry 2004 Aug 27;279(35):36841-54
Polo-like kinase 1 is overexpressed in prostate cancer and linked to higher tumor grades.
Weichert W, Schmidt M, Gekeler V, Denkert C, Stephan C, Jung K, Loening S, Dietel M, Kristiansen G
The Prostate 2004 Aug 1;60(3):240-5
The Prostate 2004 Aug 1;60(3):240-5
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various PLK1 mutation constructs.Blots were reacted with PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069, panel A) and pan reactive anti-PLK1 (panel B).Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting.Using a 1:1000 dilution, PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) detects a single band corresponding to endogenousplk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in thesemutants.Personal communication Hai Jiang, Northwestern Univ.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis is shown using PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) to detect endogenousprotein present in a Mouse A-20 whole cell lysate (arrowhead).Comparison to amolecular weight marker indicates a band of ~68 KDa corresponding to PLK1 protein.It is suggested to use a nuclear extract fromsynchronized cells to greatly increase theabundance of this protein in preparations.The blot was incubated with a 1 : 500 dilutionof the antibody at room temperaturefollowed by detection using standard techniques.Personal communication Steven Pelech, Kinexus Inc. Vancouver, BC.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) was used at a 1 : 200 dilution to detect PLK1 by immunohistochemistry in human breast carcinoma tissue.Tissue was formalin-fixed and paraffinembedded.Personal Communication, Alan Yen,Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) was used at a 1 : 200 dilution to detect PLK1 by immunohistochemistry in human colon carcinoma tissue.Tissue was formalin-fixed and paraffin embedded.Personal Communication, Alan Yen,Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)