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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
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- Product number
- GTX113833 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113833, RRID:AB_2037951
- Product name
- SDHB antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Transketolase Regulates the Metabolic Switch to Control Breast Cancer Cell Metastasis via the α-Ketoglutarate Signaling Pathway.
IBA57 mutations abrogate iron-sulfur cluster assembly leading to cavitating leukoencephalopathy.
The short variant of the mitochondrial dynamin OPA1 maintains mitochondrial energetics and cristae structure.
Tseng CW, Kuo WH, Chan SH, Chan HL, Chang KJ, Wang LH
Cancer research 2018 Jun 1;78(11):2799-2812
Cancer research 2018 Jun 1;78(11):2799-2812
IBA57 mutations abrogate iron-sulfur cluster assembly leading to cavitating leukoencephalopathy.
Ishiyama A, Sakai C, Matsushima Y, Noguchi S, Mitsuhashi S, Endo Y, Hayashi YK, Saito Y, Nakagawa E, Komaki H, Sugai K, Sasaki M, Sato N, Nonaka I, Goto YI, Nishino I
Neurology. Genetics 2017 Oct;3(5):e184
Neurology. Genetics 2017 Oct;3(5):e184
The short variant of the mitochondrial dynamin OPA1 maintains mitochondrial energetics and cristae structure.
Lee H, Smith SB, Yoon Y
The Journal of biological chemistry 2017 Apr 28;292(17):7115-7130
The Journal of biological chemistry 2017 Apr 28;292(17):7115-7130
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- SDHB antibody detects SDHB protein by western blot analysis.A. 30 ?g NCI-H929 whole cell lysate/extract12% SDS-PAGESDHB antibody (GTX113833) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse brain 12% SDS PAGE GTX113833 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- SDHB antibody detects SDHB protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: SDHB protein stained by SDHB antibody (GTX113833) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SDHB antibody detects SDHB protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in MeOH for 5 min.Green: SDHB protein stained by SDHB antibody (GTX113833) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SDHB antibody detects SDHB protein at mitochondria on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SDHB antibody (GTX113833) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SDHB antibody detects SDHB protein at mitochondria on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. SDHB antibody (GTX113833) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SDHB antibody detects SDHB protein at mitochondria on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. SDHB antibody (GTX113833) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SDHB antibody detects SDHB protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. SDHB antibody (GTX113833) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SDHB antibody detects SDHB protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. SDHB antibody (GTX113833) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SDHB antibody detects SDHB protein at mitochondria on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. SDHB antibody (GTX113833) dilution: 1:500.
