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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-21587 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SDHB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: THP-1, NCI-H929, Neuro2A.
- Concentration
- 1 mg/mL
Submitted references Characterization and metabolic synthetic lethal testing in a new model of SDH-loss familial pheochromocytoma and paraganglioma.
Smestad J, Hamidi O, Wang L, Holte MN, Khazal FA, Erber L, Chen Y, Maher LJ 3rd
Oncotarget 2018 Jan 19;9(5):6109-6127
Oncotarget 2018 Jan 19;9(5):6109-6127
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDHB Polyclonal Antibody detects SDHB protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract.12% SDS-PAGE. SDHB Polyclonal Antibody (Product # PA5-21587) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDHB Polyclonal Antibody detects SDHB protein by western blot analysis. A. 30 µg THP-1 whole cell lysate/extract. B. 30 µg NCI-H929 whole cell lysate/extract.12% SDS-PAGE. SDHB Polyclonal Antibody (Product # PA5-21587) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SDHB was achieved by transfecting HeLa with SDHB specific siRNAs (Silencer® select Product # s532300, s12654). Western blot analysis (Fig. a) was performed using Whole cell extracts from the SDHB knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SDHB Polyclonal Antibody (Product # PA5-21587, 1:1000 dilution ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SDHB.A non specific band around 65 kDa was observed
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SDHB Polyclonal Antibody(Product # PA5-21587) and a ~31kDa band corresponding to SDHB was observed across cell lines and tissues tested. Whole cell extracts (30ug µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), MCF7 (Lane 3), HEK-293 (Lane 4), Mouse Kidney (Lane 5) and Rat Kidney (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). A non specific band was observed around 65kDa across cell lines and tissues tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SDHB was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SDHB Polyclonal Antibody (Product # PA5-21587) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDHB Polyclonal Antibody detects SDHB protein at mitochindria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. SDHB Polyclonal Antibody (Product # PA5-21587) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDHB Polyclonal Antibody detects SDHB protein at mitochondria on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. SDHB Polyclonal Antibody (Product # PA5-21587) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
