- PA5-28600 - Invitrogen Antibodies TAB1 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of TAB1 using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate (C) 30 µg HeLa whole cell lysate and D) 30 µg A375 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a TAB1 polyclonal antibody (Product # PA5-28600) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot using TAB1 Polyclonal Antibody (Product # PA5-28600). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TAB1 Polyclonal Antibody (Product # PA5-28600) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: TAB1 Polyclonal Antibody detects MAP3K7IP1 protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract. B. 30 µg C8D30 whole cell lysate/extract.10% SDS-PAGE. TAB1 Polyclonal Antibody (Product # PA5-28600) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of TAB1 was achieved by transfecting HeLa with TAB1 specific siRNAs (Silencer® select Product # s20451). Western blot analysis (Fig. a) was performed using whole cell extracts from the TAB1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with TAB1 Monoclonal Antibody (Product # PA5-28600, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to TAB1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using TAB1 Monoclonal Antibody (Product # PA5-28600) and a 55 kDa band corresponding to TAB1 was observed across all the cell lines and tissues tested along with additional uncharacterized bands (*). Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), MCF-7 (Lane 3), MDA-MB-231 (Lane 4), SK-OV-3 (Lane 5), PC-12 (Lane 6), HepG2 (Lane 7), tissue extracts of Mouse Brain (Lane 8), Mouse Lung (Lane 9), Mouse Liver (Lane 10) and Mouse Heart (Lane 11) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of TAB1 in paraformaldehyde-fixed HeLa cells using a TAB1 polyclonal antibody (Product # PA5-28600) at a 1:100 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TAB1 (Product # PA5-28600) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-28600