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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-28683 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, Molt-4, Neuro 2A, C8D30, NIH-3T3, Raw264.7, PC-12, Rat2.
- Concentration
- 0.6 mg/mL
Submitted references Myeloid Differentiation Primary Response 88-Cyclin D1 Signaling in Breast Cancer Cells Regulates Toll-Like Receptor 3-Mediated Cell Proliferation.
Singh A, Devkar R, Basu A
Frontiers in oncology 2020;10:1780
Frontiers in oncology 2020;10:1780
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TAB1 using 30 µg BCL-1 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a TAB1 polyclonal antibody (Product # PA5-28683) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TAB1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TAB1 Polyclonal Antibody (Product # PA5-28683) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TAB1 Polyclonal Antibody (Product # PA5-28683). Sample (30 µg whole cell lysate). A: A431. B: MOLT4. 7.5% SDS PAGE. TAB1 Polyclonal Antibody (Product # PA5-28683) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TAB1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TAB1 Polyclonal Antibody (Product # PA5-28683) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TAB1 in paraformaldehyde-fixed HeLa cells using a TAB1 polyclonal antibody (Product # PA5-28683) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Western blotting for the expression of signaling protein. (A,B) Cell lysate were collected and subjected to Western blot assay to estimate the level of expression of interleukin 1 receptor-associated kinase 1 (IRAK1), transforming growth factor beta-activated kinase 1 (TAK1), TGF-beta-activated kinase 1 (TAB1), TNF receptor-associated factor 6 (TRAF6), and cyclin D1. (C,D) Expression of pIRAK1 and pTAK1. beta-actin was used as loading control. The respective bar graphs are presented as densitometry analysis as mean +- SD of experiments ( p < 0.05 is treated as significant).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Immunoprecipitation showing the involvement of the signaling complex. (A) Signaling complex of IRAK1/TRAF6 was immunoprecipitated with antibodies against IRAK1 followed by Western blotting with anti-TRAF6 and anti-IRAK1 antibodies. (B) Signaling complex of pIRAK1/TAK1 was immunoprecipitated with antibodies against pIRAK1 followed by Western blotting with anti-TAK1 and anti-pIRAK1 antibodies. (C) Signaling complex TAB1-TRAF6-TAK1 was immunoprecipitated with antibodies against pTAK1 followed by Western blotting using anti-TRAF6, TAB1, and pTAK1 antibodies. (D) Signaling complex TAB1-TRAF6-TAK1 was immunoprecipitated with antibodies against TRAF6 followed by Western blotting analysis using anti-TAK1, TAB1, and TRAF6 antibodies.
