PA5-116630

antibody from Invitrogen Antibodies

Targeting: POLRMT APOLMT, h-mtRPOL, MTRNAP, MTRPOL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-116630

Provider

Invitrogen Antibodies

Product name

POLRMT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: HeLa, HepG2, 293T

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.74 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of POLRMT using POLRMT Polyclonal Antibody (Product # PA5-116630) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of POLRMT in C6 cells using POLRMT Polyclonal Antibody (Product # PA5-116630) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of POLRMT in U-2 OS cells using POLRMT Polyclonal Antibody (Product # PA5-116630) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of POLRMT in 200 µg extracts of HepG2 cells using POLRMT Polyclonal Antibody (Product # PA5-116630). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:100

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of POLRMT in 200 µg extracts of HepG2 cells using POLRMT Polyclonal Antibody (Product # PA5-116630). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:100