Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB7750 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Lgr4/GPR48 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Lgr4/GPR48 in Lgr4-transfected cell-based ELISA. Stains human Lgr4/GPR48 transfected but not irrelevant transfected cells in flow cytometry.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9BXB1- Isotype
- IgG
- Antibody clone number
- 852229
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Lgr4/GPR48 in HT-29 Human Cell Line. Lgr4/GPR48 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human Lgr4/GPR48 Monoclonal Antibody (Catalog # MAB7750) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Lgr4/GPR48 in HEK293 Human Cell Line Transfected with Human Lgr4/GPR48 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Lgr4/GPR48 and eGFP was stained with and either (A) Mouse Anti-Human Lgr4/GPR48 Monoclonal Antibody (Catalog # MAB7750) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- R-Spondin 4 Binding to Lgr4/GPR48-transfected HEK293 Human Cell Line is Blocked by Human Lgr4/GPR48 Antibody. In a functional flow cytometry test, biotinylated Recombinant Human R-Spondin 4 (100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with human Lgr4/GPR48 (dark orange histogram). Binding is completely blocked (light orange histogram) by 2.5 μg/mL of Mouse Anti-Human Lgr4/GPR48 Monoclonal Antibody (Catalog # MAB7750). Mouse IgG2B Isotype Control (Catalog # MAB004) at 2.5 μg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050).
