Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP32978PU-N - Provider product page
- Provider
- OriGene
- Product name
- GPR48 (LGR4) rat monoclonal antibody, clone BBX-1H1, Purified
- Antibody type
- Monoclonal
- Description
- GPR48 (LGR4) rat monoclonal antibody, clone BBX-1H1, Purified
- Host
- Rat
- Conjugate
- Unconjugated
- Epitope
- LGR4
- Isotype
- IgG
- Antibody clone number
- BBX-1H1
- Vial size
- 100 µg
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified BBX-1H1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified BBX-1H1 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using BBX-1H1 Cat.-No?AP32978PU-N. BOSC23 cells were transiently transfected with an expression vector encoding either LGR4-hum (red curve), LGR4-murine (blue curve) or an irrelevant protein (control transfectant: black curve). Binding of BBX-3A8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with LGR4-hum transfected cells.
- Validation comment
- FC