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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [3]
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- Product number
- R32476 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CCR1 Antibody
- Antibody type
- Polyclonal (Antigen purified)
- Description
- Antigen affinity purified
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- Prior to reconstitution, store at 4°C. After reconstitution, the CCR1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat brain and 2) mouse brain lysate with CCR1 antibody at 0.5ug/ml. Expected molecular weight: 40-45 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse lymph tissue with CCR1 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat spleen tissue with CCR1 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse RAW264.7 cells with CCR1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CCR1 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse SP2/0 cells with CCR1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CCR1 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse YAC-1 cells with CCR1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CCR1 antibody.
