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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00000103-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000103-B01P, RRID:AB_1137568
- Product name
- ADAR purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human ADAR protein.
- Antigen sequence
MNPRQGYSLSGYYTHPFQGYEHRQLRYQQPGPGSS
PSSFLLKQIEFLKGQLPEAPVIGKQTPSLPPSLPG
LRPRFPVLLASSTRGRQVDIRGVPRGVHLGSQGLQ
RGFQHPSPRGRSLPQRGVDCLSSHFQELSIYQDQE
QRILKFLEELGEGKATTAHDLSGKLGTPKKEINRV
LYSLAKKGKLQKEAGTPPLWKIAVSTQAWNQHSGV
VRPDGHSQGAPNSDPSLEPEDRNSTSVSEDLLEPF
IAVSAQAWNQHSGVVRPDSHSQGSPNSDPGLEPED
SNSTSALEDPLEFLDMAEIKEKICDYLFNVSDSSA
LNLAKNIGLTKARDINAVLIDMERQGDVYRQGTTP
PIWHLTDKKRERMQIKRNTNSVPETAPAAIPETRR
NAEFLTCNIPTSNASNNMVTTEKVENGQEPVIKLE
NRQEARPEPARLKPPVHYNGPSKAGYVDFENGQWA
TDDIPDDLNSIRAAPGEFRAIMEMPSFYSHGLPRC
SPYKKLTECQLKNPISGLLEYAQFASQTCEFNMIE
QSGPPHEPRFKFQVVINGREFPPAEAGSKKVAKQD
AAMKAMTILLEEAKAKDSGKSEESSHYSTEKESEK
TAESQTPTPSATSFFSGKSPVTTLLECMHKLGNSC
EFRLLSKEGPAHEPKFQYCVAVGAQTFPSVSAPSK
KVAKQMAAEEAMKALHGEATNSMASDNQPEGMISE
SLDNLESMMPNKVRKIGELVRYLNTNPVGGLLEYA
RSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFP
AVCAHSKKQGKQEAADAALRVLIGENEKAERMGFT
EVTPVTGASLRRTMLLLSRSPEAQPKTLPLTGSTF
HDQIAMLSHRCFNTLTNSFQPSLLGRKILAAIIMK
KDSEDMGVVVSLGTGNRCVKGDSLSLKGETVNDCH
AEIISRRGFIRFLYSELMKYNSQTAKDSIFEPAKG
GEKLQIKKTVSFHLYISTAPCGDGALFDKSCSDRA
MESTESRHYPVFENPKQGKLRTKVENGEGTIPVES
SDIVPTWDGIRLGERLRTMSCSDKILRWNVLGLQG
ALLTHFLQPIYLKSVTLGYLFSQGHLTRAICCRVT
RDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGK
TKETSVNWCLADGYDLEILDGTRGTVDGPRNELSR
VSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAAR
DYETAKNYFKKGLKDMGYGNWISKPQEEKNFYLCP
V- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references ADAR2-mediated editing of miR-214 and miR-122 precursor and antisense RNA transcripts in liver cancers.
Liu WH, Chen CH, Yeh KH, Li CL, Wu YJ, Chen DS, Chen PJ, Yeh SH
PloS one 2013;8(12):e81922
PloS one 2013;8(12):e81922
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ADAR MaxPab polyclonal antibody. Western Blot analysis of ADAR expression in human liver.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ADAR MaxPab polyclonal antibody. Western Blot analysis of ADAR expression in HepG2.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ADAR expression in transfected 293T cell line (H00000103-T01) by ADAR MaxPab polyclonal antibody.Lane 1: ADAR transfected lysate(134.86 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to ADAR on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of purified MaxPab antibody to ADAR on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
