HPA003890

antibody from Atlas Antibodies

Targeting: ADAR ADAR1, G1P1, IFI4

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA003890

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003890, RRID:AB_1078103

Product name

Anti-ADAR

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

SDNQPEGMISESLDNLESMMPNKVRKIGELVRYLN
TNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFV
YQAKVGGRWFPAVCAHSKKQGKQEAADAALRVLIG
ENEKAERMGFTEVTPVTGASLRRTMLLLSRSPEA


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

MicroRNA editing facilitates immune elimination of HCMV infected cells.

Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le VT, Galun E, Horejsi V, Isakov O, Shomron N, Wolf DG, Hengel H, Mandelboim O
PLoS pathogens 2014 Feb;10(2):e1003963

---

ADAR-Related Activation of Adenosine-to-Inosine RNA Editing During Regeneration

Witman N, Behm M, Öhman M, Morrison J
Stem Cells and Development 2013 August;22(16):2254-2267

---

Mutations in ADAR1 cause Aicardi-Goutières syndrome associated with a type I interferon signature.

Rice GI, Kasher PR, Forte GM, Mannion NM, Greenwood SM, Szynkiewicz M, Dickerson JE, Bhaskar SS, Zampini M, Briggs TA, Jenkinson EM, Bacino CA, Battini R, Bertini E, Brogan PA, Brueton LA, Carpanelli M, De Laet C, de Lonlay P, del Toro M, Desguerre I, Fazzi E, Garcia-Cazorla A, Heiberg A, Kawaguchi M, Kumar R, Lin JP, Lourenco CM, Male AM, Marques W Jr, Mignot C, Olivieri I, Orcesi S, Prabhakar P, Rasmussen M, Robinson RA, Rozenberg F, Schmidt JL, Steindl K, Tan TY, van der Merwe WG, Vanderver A, Vassallo G, Wakeling EL, Wassmer E, Whittaker E, Livingston JH, Lebon P, Suzuki T, McLaughlin PJ, Keegan LP, O'Connell MA, Lovell SC, Crow YJ
Nature genetics 2012 Nov;44(11):1243-8

Western blot

Enhanced validation

Submitted by

klas2

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting ADAR or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting ADAR Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control

Sample type

U-2 OS

Primary Ab dilution

1:196

Conjugate

Horseradish Peroxidase

Secondary Ab

Secondary Ab

Secondary Ab dilution

1:3000

Knockdown/Genetic Approaches Application

Western blot

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no

s1007

KD Reagent Provider

Thermo Fisher Scientific

KD Reagent Prod no 2

s1008

KD Reagent Page 2

KD Reagent Page 2

KD Reagent Prod no 3

s1009

KD Reagent Page 3

KD Reagent Page 3

Antibody Lot Number

R04101

Appendix

565c9c16e5d38.pdf

Show more

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ADAR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ADAR antibody. Remaining relative intensity is presented

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex, colon, kidney and liver using Anti-ADAR antibody HPA003890 (A) shows similar protein distribution across tissues to independent antibody HPA051519 (B).

Antibody #2 product nr

HPA051519

Antibody provider

Atlas Antibodies

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and subset of glial cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver using Anti-ADAR antibody HPA003890.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-ADAR antibody HPA003890.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney using Anti-ADAR antibody HPA003890.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex using Anti-ADAR antibody HPA003890.

Sample type

HUMAN