LS-C818758

antibody from LSBio

Targeting: IFITM1 9-27, CD225, DSPA2a, IFI17

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C818758

Provider

LSBio

Product name

CD225 / IFITM1 Antibody (clone 021, Preservative Free) LS-C818758

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

021

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

CD225 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 2 uL anti-CD225 rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CD225 rabbit monoclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CD225 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 2 uL anti-CD225 rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CD225 rabbit monoclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CD225 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 2 uL anti-CD225 rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CD225 rabbit monoclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa.