Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- A00808 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-IL-21R (ED) Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal antibody for IL21R detection. Tested positive for IHC, WB in Human, Mouse, Rat
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100µg
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- +4°C
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of IL21R using anti-IL21R antibody (A00808).IL21R was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IL21R Antibody (A00808) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image