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Learn10533-1-AP
antibody from Invitrogen Antibodies
Targeting: IL21R
CD360
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 10533-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL21R Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LLLLLLVIV FIPAFWSLKT HPLWRLWKKI WAVPSPERFF MPLYKGCSGD FKKWVGAPFT GSSLELGPWS PEVPSTLEVY SCHPPRSPAK RLQLTELQEP AELVESDGVP KPSFWPTAQN SGGSAYSEER DRPYGLVSID TVTVLDAEGP CTWPCSCEDD GYPALDLDAG LEPSPGLEDP LLDAGTTVLS CGCVSAGSPG LGGPLGSLLD RLKPPLADGE DWAGGLPWGG RSPGGVSESE AGSPLAGLDM DTFDSGFVGS DCSSPVECDF TSPGDEGPPR SYLRQWVVIP PPLSSPGPQA S (239-538 aa encoded by BC004348)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.37 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COLO 320 cells using 10533-1-AP (IL21R antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 10533-1-AP ( IL21R Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 10533-1-AP ( IL21R Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 K-562 cells were stained with 0.2ug IL21R antibody (10533-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-IL21R (IP:10533-1-AP, 4ug; Detection:10533-1-AP 1:1000) with COLO 320 cells lysate 2000ug.
