Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX84288 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84288, RRID:AB_10728529
- Product name
- IRF3 antibody [4D4]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IRF3 monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-IRF3 mouse monoclonal antibody (GTX84288) immunofluorescent staining of COS7 cells transiently transfected with IRF3
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-IRF3mouse monoclonal antibody. (GTX84288, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either IRF3 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IRF3 antibody (GTX84288), and then analyzed by flow cytometry.
