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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF1956 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Crossveinless-2/CV-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Crossveinless-2/CV-2 in direct ELISAs and Western blots. In these formats, approximately 10% cross-reactivity with recombinant mouse CV-2 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q8N8U9- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references BMP activity controlled by BMPER regulates the proinflammatory phenotype of endothelium.
Helbing T, Rothweiler R, Ketterer E, Goetz L, Heinke J, Grundmann S, Duerschmied D, Patterson C, Bode C, Moser M
Blood 2011 Nov 3;118(18):5040-9
Blood 2011 Nov 3;118(18):5040-9
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Crossveinless-2/CV-2 in HUVEC Human Cells. Crossveinless-2/CV-2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Human Crossveinless-2/CV-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1956) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
