IC646P

antibody from R&D Systems

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC646P

Provider

R&D Systems

Product name

Human IL-18/IL-1F4 Propeptide PE-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects the pro region of human IL-18/IL-1F4 in direct ELISAs and Western blots. Does not cross-react with recombinant human IL-18, recombinant mouse IL-18 or recombinant rat IL-18.

Reactivity

Human

Host

Mouse

Antigen sequence

Q14116


Isotype

IgG

Antibody clone number

74801

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Altered expression of IL-18 binding protein and IL-18 receptor in basophils and mast cells of asthma patients.

Wang Z, Liu Z, Wang L, Wang J, Chen L, Xie H, Zhang H, He S
Scandinavian journal of immunology 2018 May;87(5):e12658

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Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R.

Zhang H, Wang J, Wang L, Xie H, Chen L, He S
Journal of cellular and molecular medicine 2018 Jan;22(1):354-373

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Human CD8⁺ EMRA T cells display a senescence-associated secretory phenotype regulated by p38 MAPK.

Callender LA, Carroll EC, Beal RWJ, Chambers ES, Nourshargh S, Akbar AN, Henson SM
Aging cell 2018 Feb;17(1)

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Enhanced Expression of IL-18 and IL-18BP in Plasma of Patients with Eczema: Altered Expression of IL-18BP and IL-18 Receptor on Mast Cells.

Hu Y, Wang J, Zhang H, Xie H, Song W, Jiang Q, Zhao N, He S
Mediators of inflammation 2017;2017:3090782

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of IL-18/IL-1F4 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (upper panel) or treated with LPS for 24 hours (lower panel) were stained with Mouse Anti-Human IL-18/IL-1F4 PE-conjugated Monoclonal Antibody (Catalog # IC646P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.