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- Antibody Data
- Antigen structure
- References [3]
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- Flow cytometry [2]
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- Product number
- MAB646 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-18/IL-1F4 Propeptide Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects the pro region of human IL-18/IL-1F4 in direct ELISAs and Western blots. Does not cross-react with recombinant human IL-18, recombinant mouse IL-18 or recombinant rat IL-18.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q14116- Isotype
- IgG
- Antibody clone number
- 74801
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis.
IL-18 Is Involved in Eosinophil-Mediated Tumoricidal Activity against a Colon Carcinoma Cell Line by Upregulating LFA-1 and ICAM-1.
IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
Li WL, Hua LG, Qu P, Yan WH, Ming C, Jun YD, Yuan LD, Nan N
Archives of medical science : AMS 2016 Oct 1;12(5):950-958
Archives of medical science : AMS 2016 Oct 1;12(5):950-958
IL-18 Is Involved in Eosinophil-Mediated Tumoricidal Activity against a Colon Carcinoma Cell Line by Upregulating LFA-1 and ICAM-1.
Gatault S, Delbeke M, Driss V, Sarazin A, Dendooven A, Kahn JE, Lefèvre G, Capron M
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2483-92
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2483-92
IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
Reeves EP, Williamson M, Byrne B, Bergin DA, Smith SG, Greally P, O'Kennedy R, O'Neill SJ, McElvaney NG
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 1;184(3):1642-52
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 1;184(3):1642-52
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-18/IL-1F4 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (upper panel) or treated with LPS for 24 hours (lower panel) were stained with Mouse Anti-Human IL-18/IL-1F4 Propeptide Monoclonal Antibody (Catalog # MAB646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-18/IL-1F4 Propeptide in Human THP-1 cells by Flow Cytometry. THP-1 human acute monocytic leukemia cells were stained with Mouse Anti-Human IL-18/IL-1F4 Propeptide Monoclonal Antibody (Catalog # MAB646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
