12455-1-AP

antibody from Invitrogen Antibodies

Targeting: EPS8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12455-1-AP

Provider

Invitrogen Antibodies

Product name

EPS8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: GMYPSQMNG YGSSPTFSQT DREHGSKTSA KALYEQRKNY ARDSVSSVSD ISQYRVEHLT TFVLDRKDAM ITVDDGIRKL KLLDAKGKVW TQDMILQVDD RAVSLIDLES KNELENFPLN TIQHCQAVMH SCSYDSVLAL VCKEPTQNKP DLHLFQCDEV KANLISEDIE SAISDSKGGK QKRRPDALRM ISNADPSIPP PPRAPAPAPP GTVTQVDVRS RVAAWSAWAA DQGDFEKPRQ YHEQEETPEM MAARID (13-267 aa encoded by BC030010)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.49 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12455-1-AP (EPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 12455-1-AP (EPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A375 cells using 12455-1-AP (EPS8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A375 cells using 12455-1-AP (EPS8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12455-1-AP ( EPS8 antibody) at dilution of 1:100 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12455-1-AP ( EPS8 antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EPS8 (IP:12455-1-AP, 3ug; Detection:12455-1-AP 1:500) with HeLa cells lysate 3800ug.