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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-9966 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-9966, RRID:AB_627235
- Product name
- Anti-MCM7
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Pre-replication complex proteins assemble at regions of low nucleosome occupancy within the Chinese hamster dihydrofolate reductase initiation zone.
RB deletion disrupts coordination between DNA replication licensing and mitotic entry in vivo.
Oxidative stress accumulates in adipose tissue during aging and inhibits adipogenesis.
Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis
Mitotic chromosome condensation mediated by the retinoblastoma protein is tumor-suppressive.
A Dominant-Negative PPARgamma Mutant Promotes Cell Cycle Progression and Cell Growth in Vascular Smooth Muscle Cells.
Recruitment of fanconi anemia and breast cancer proteins to DNA damage sites is differentially governed by replication.
Replication licensing promotes cyclin D1 expression and G1 progression in untransformed human cells.
Plx1 is required for chromosomal DNA replication under stressful conditions.
Responses of genes involved in cell cycle control to diverse DNA damaging chemicals in human lung adenocarcinoma A549 cells.
Lubelsky Y, Sasaki T, Kuipers MA, Lucas I, Le Beau MM, Carignon S, Debatisse M, Prinz JA, Dennis JH, Gilbert DM
Nucleic acids research 2011 Apr;39(8):3141-55
Nucleic acids research 2011 Apr;39(8):3141-55
RB deletion disrupts coordination between DNA replication licensing and mitotic entry in vivo.
Bourgo RJ, Ehmer U, Sage J, Knudsen ES
Molecular biology of the cell 2011 Apr;22(7):931-9
Molecular biology of the cell 2011 Apr;22(7):931-9
Oxidative stress accumulates in adipose tissue during aging and inhibits adipogenesis.
Findeisen HM, Pearson KJ, Gizard F, Zhao Y, Qing H, Jones KL, Cohn D, Heywood EB, de Cabo R, Bruemmer D
PloS one 2011 Apr 14;6(4):e18532
PloS one 2011 Apr 14;6(4):e18532
Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis
Yoshitami Hashimoto, Arnab Ray Chaudhuri, Massimo Lopes, Vincenzo Costanzo
Nature Structural & Molecular Biology 2010 Oct;17(11):1305-1311
Nature Structural & Molecular Biology 2010 Oct;17(11):1305-1311
Mitotic chromosome condensation mediated by the retinoblastoma protein is tumor-suppressive.
Coschi CH, Martens AL, Ritchie K, Francis SM, Chakrabarti S, Berube NG, Dick FA
Genes & development 2010 Jul 1;24(13):1351-63
Genes & development 2010 Jul 1;24(13):1351-63
A Dominant-Negative PPARgamma Mutant Promotes Cell Cycle Progression and Cell Growth in Vascular Smooth Muscle Cells.
Liu JZ, Lyon CJ, Hsueh WA, Law RE
PPAR research 2009;2009:438673
PPAR research 2009;2009:438673
Recruitment of fanconi anemia and breast cancer proteins to DNA damage sites is differentially governed by replication.
Shen X, Do H, Li Y, Chung WH, Tomasz M, de Winter JP, Xia B, Elledge SJ, Wang W, Li L
Molecular cell 2009 Sep 11;35(5):716-23
Molecular cell 2009 Sep 11;35(5):716-23
Replication licensing promotes cyclin D1 expression and G1 progression in untransformed human cells.
Liu P, Slater DM, Lenburg M, Nevis K, Cook JG, Vaziri C
Cell cycle (Georgetown, Tex.) 2009 Jan 1;8(1):125-36
Cell cycle (Georgetown, Tex.) 2009 Jan 1;8(1):125-36
Plx1 is required for chromosomal DNA replication under stressful conditions.
Trenz K, Errico A, Costanzo V
The EMBO journal 2008 Mar 19;27(6):876-85
The EMBO journal 2008 Mar 19;27(6):876-85
Responses of genes involved in cell cycle control to diverse DNA damaging chemicals in human lung adenocarcinoma A549 cells.
Zhu H, Smith C, Ansah C, Gooderham NJ
Cancer cell international 2005 Aug 24;5:28
Cancer cell international 2005 Aug 24;5:28
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 7
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
