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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB3278 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat TRAF-3 Isoform 2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human, mouse, and rat TRAF-3 in Western blots. In Western blots, this antibody does not cross-react with recombinant human TRAF-1, -2, -4, -5, or -6.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
NP_663778- Isotype
- IgG
- Antibody clone number
- 433113
- Vial size
- 250 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human/Mouse/Rat TRAF-3 Isoform 2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse/Rat TRAF-3 Isoform 2 Monoclonal Antibody (Catalog # MAB3278) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TRAF-3 Isoform 2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
