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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- DM3525 - Provider product page
- Provider
- OriGene
- Product name
- PLGF (PGF) (PlGF-1/2) mouse monoclonal antibody, clone 178/G10, Purified
- Antibody type
- Monoclonal
- Description
- PLGF (PGF) (PlGF-1/2) mouse monoclonal antibody, clone 178/G10, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PGF
- Isotype
- IgG
- Antibody clone number
- 178/G10
- Vial size
- 100 µg
- Concentration
- Lyophilized
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis: Detection of PlGF in Human placenta lysates. a) Patient 1 b) Patient 2 (50 ug lysate/lane). (178/G10) was used at 2 ug/ml as first antibody. AP-conjugated Goat anti Mouse was used as a secondary antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- PlGF Sandwich-ELISA using recombinant human PlGF-1 as?standard (?DA3508). Mouse anti-Human PlGF #178/G10 ?was used as capture antibody, Biotinylated rabbit anti-human PlGF was used for detection.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence with anti-Human PlGF antibodies (clones G10 and 3B10): Sf9 insect cells were infected with recombinant Baculovirus for Human PlGF-1 and PlGF-2. Three days after infection the Immunofluorescence protocol was performed.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunprecipitation of rh-PlGF-1 using monoclonal anti-Human PlGF antibody (clone 178/G10 )
- Validation comment
- IP
