DM3525
antibody from Acris Antibodies GmbH
Targeting: PGF
D12S1900, PGFL, PIGF, PLGF, PlGF-2, SHGC-10760
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- DM3525 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#DM3525, RRID:AB_1770607
- Product name
- anti Placenta growth factor / PGF (PlGF-1/2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human PlGF-2 [Leu19 â Arg170]Â (produced in insect cells)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 178/G10
- Vial size
- 0.1 mg
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: Detection of PlGF in Human placenta lysates.a) Patient 1b) Patient 2 (50 µg lysate/lane).DM3525 (178/G10) was used at 2 µg/ml as first antibody. AP-conjugated Goat anti Mouse was used as a secondary antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- PlGF Sandwich-ELISA using recombinant human PlGF-1 as standard (Cat.-No DA3508). Mouse anti-Human PlGF #178/G10 (Cat.-No DM3525) was used as capture antibody, Biotinylated rabbit anti-human PlGF was used for detection.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence with anti-Human PlGF antibodies (clones G10 and 3B10): Sf9 insect cells were infected with recombinant Baculovirus for Human PlGF-1 and PlGF-2. Three days after infection the Immunofluorescence protocol was performed.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunprecipitation of rh-PlGF-1 using monoclonal anti-Human PlGF antibody (clone 178/G10 Cat#DM3525)