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LearnGTX105278
antibody from GeneTex
Targeting: ZEB1
AREB6, BZP, FECD6, NIL-2-A, PPCD3, TCF8, ZEB, Zfhep, Zfhx1a
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [3]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX105278 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105278, RRID:AB_11162905
- Product name
- ZEB1 antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
PEGylated liposome-encapsulated rhenium-188 radiopharmaceutical inhibits proliferation and epithelial-mesenchymal transition of human head and neck cancer cells in vivo with repeated therapy.
Overexpression of CD44 Standard Isoform Upregulates HIF-1α Signaling in Hypoxic Breast Cancer Cells.
TOP2A induces malignant character of pancreatic cancer through activating β-catenin signaling pathway.
A TP73-AS1/miR-200a/ZEB1 regulating loop promotes breast cancer cell invasion and migration.
A novel long non-coding RNA linc-ZNF469-3 promotes lung metastasis through miR-574-5p-ZEB1 axis in triple negative breast cancer.
A stemness-related ZEB1-MSRB3 axis governs cellular pliancy and breast cancer genome stability.
Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
MiR-206 suppresses epithelial mesenchymal transition by targeting TGF-β signaling in estrogen receptor positive breast cancer cells.
Pei YF, Xu XN, Wang ZF, Wang FW, Wu WD, Geng JF, Liu XQ
The American journal of pathology 2019 Feb 5;
The American journal of pathology 2019 Feb 5;
PEGylated liposome-encapsulated rhenium-188 radiopharmaceutical inhibits proliferation and epithelial-mesenchymal transition of human head and neck cancer cells in vivo with repeated therapy.
Chang CY, Chen CC, Lin LT, Chang CH, Chen LC, Wang HE, Lee TW, Lee YJ
Cell death discovery 2018;4:100
Cell death discovery 2018;4:100
Overexpression of CD44 Standard Isoform Upregulates HIF-1α Signaling in Hypoxic Breast Cancer Cells.
Ryu D, Ryoo IG, Kwak MK
Biomolecules & therapeutics 2018 Sep 1;26(5):487-493
Biomolecules & therapeutics 2018 Sep 1;26(5):487-493
TOP2A induces malignant character of pancreatic cancer through activating β-catenin signaling pathway.
Pei YF, Yin XM, Liu XQ
Biochimica et biophysica acta. Molecular basis of disease 2018 Jan;1864(1):197-207
Biochimica et biophysica acta. Molecular basis of disease 2018 Jan;1864(1):197-207
A TP73-AS1/miR-200a/ZEB1 regulating loop promotes breast cancer cell invasion and migration.
Zou Q, Zhou E, Xu F, Zhang D, Yi W, Yao J
Journal of cellular biochemistry 2018 Feb;119(2):2189-2199
Journal of cellular biochemistry 2018 Feb;119(2):2189-2199
A novel long non-coding RNA linc-ZNF469-3 promotes lung metastasis through miR-574-5p-ZEB1 axis in triple negative breast cancer.
Wang PS, Chou CH, Lin CH, Yao YC, Cheng HC, Li HY, Chuang YC, Yang CN, Ger LP, Chen YC, Lin FC, Shen TL, Hsiao M, Lu PJ
Oncogene 2018 Aug;37(34):4662-4678
Oncogene 2018 Aug;37(34):4662-4678
A stemness-related ZEB1-MSRB3 axis governs cellular pliancy and breast cancer genome stability.
Morel AP, Ginestier C, Pommier RM, Cabaud O, Ruiz E, Wicinski J, Devouassoux-Shisheboran M, Combaret V, Finetti P, Chassot C, Pinatel C, Fauvet F, Saintigny P, Thomas E, Moyret-Lalle C, Lachuer J, Despras E, Jauffret JL, Bertucci F, Guitton J, Wierinckx A, Wang Q, Radosevic-Robin N, Penault-Llorca F, Cox DG, Hollande F, Ansieau S, Caramel J, Birnbaum D, Vigneron AM, Tissier A, Charafe-Jauffret E, Puisieux A
Nature medicine 2017 May;23(5):568-578
Nature medicine 2017 May;23(5):568-578
Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Wang LT, Chiou SS, Chai CY, Hsi E, Chiang CM, Huang SK, Wang SN, Yokoyama KK, Hsu SH
Oncogene 2017 Aug;36(31):4405-4414
Oncogene 2017 Aug;36(31):4405-4414
The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
Chiu WC, Lee YC, Su YH, Wang YY, Tsai CH, Hou YA, Wang CH, Huang YF, Huang CJ, Chou SH, Hsieh PW, Yuan SF
PloS one 2016;11(11):e0166453
PloS one 2016;11(11):e0166453
MiR-206 suppresses epithelial mesenchymal transition by targeting TGF-β signaling in estrogen receptor positive breast cancer cells.
Yin K, Yin W, Wang Y, Zhou L, Liu Y, Yang G, Wang J, Lu J
Oncotarget 2016 Apr 26;7(17):24537-48
Oncotarget 2016 Apr 26;7(17):24537-48
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: HeLa 5% SDS PAGE GTX105278 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody (GTX105278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZEB1(GTX105278) antibody at 1:1000 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ZEB1 antibody [N2C1], Internal detects ZEB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ZEB1 protein stained by ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar = 10 £gm.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ZEB1 antibody [N2C1], Internal detects ZEB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ZEB1 protein stained by ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ZEB1 antibody immunoprecipitates ZEB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 £gg 293T whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of ZEB1 protein by 2 £gg of ZEB1 antibody (GTX105278) 7.5% SDS-PAGE The immunoprecipitated ZEB1 protein was detected by ZEB1 antibody (GTX105278) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 £gg of either control rabbit IgG or anti-ZEB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.
