Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-88758 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ITPR3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EGSEAYQRCE SGGFLSKLIQ HTKDLMESEE KLCIKVLRTL QQMLLKKTKY GDRGNQLRKM LLQNYLQNRK STSRGDLPDP IGTGLDPDWS AIAATQCRLD KEGATKLVCD LITSTKNEKI FQESIGLAIH LLDGGNTEIQ KSFHNLMMSD KKSERFFKVL HDRMKRAQQE TKSTVAVNMN DLGSQPHEDR EPVDPTTKGR VASFSIPGSS SRYSLGPSLR RGHEVSERVQ SSEMGTSVLI M; Positive Samples: HeLa, LO2, HepG2; Cellular Location: Endoplasmic reticulum membrane, Multi-pass membrane protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.34 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ITPR3 in extracts of various cell lines using ITPR3 Polyclonal Antibody (Product # PA5-88758) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ITPR3 in extracts of various cell lines using ITPR3 Polyclonal Antibody (Product # PA5-88758) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ITPR3 was performed in NIH-3T3 cells using ITPR3 Polyclonal Antibody (Product # PA5-88758) at a dilution of 1:100. Blue: DAPI for nuclear staining.
