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LearnMA5-19703
antibody from Invitrogen Antibodies
Targeting: PKN1
DBK, MGC46204, PAK1, PKN, PRK1, PRKCL1
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-19703 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKN1 Monoclonal Antibody (1B10)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Peptide Sequence: LDMEPQGCLV AEVTFRNPVI ERIPRLRRQK KIFSKQQGKA FQRARQMNID VATWVRLLRR LIPNATGTGT FSPGASPGSE ARTTGDISVE KLNLGTDSDS SPQKSSRDPP SSPSSLSSPI QESTAPELPS ETQETPGPAL CSPLRKSPLT LEDF
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1B10
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PKN1 expressed in transfected 293T cell line by anti-PKN1 monoclonal antibody (Product # MA5-19703), clone 1B10. Lane 1: PKN1 transfected lysate (103.9 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PKN1 over-expressed 293 cell line, cotransfected with PKN1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PKN1 monoclonal antibody clone 1B10 (Product # MA5-19703). GAPDH (36.1 kDa) used as specificity and loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PKN1 using anti-PKN1 monoclonal antibody (Product # MA5-19703) is approximately 3 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using an anti-PKN1 monoclonal antibody (Product # MA5-19703). (antibody concentration 10 µg/mL).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PKN1 transfected lysate using anti-PKN1 monoclonal antibody (Product # MA5-19703) and Protein A Magnetic Beads, and immunoblotted with PKN1 monoclonal antibody (Product # MA5-19703).
