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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- GTX114781 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114781, RRID:AB_10620693
- Product name
- BAF57 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references The E3 ubiquitin ligase activity of Trip12 is essential for mouse embryogenesis.
Kajiro M, Tsuchiya M, Kawabe Y, Furumai R, Iwasaki N, Hayashi Y, Katano M, Nakajima Y, Goto N, Watanabe T, Murayama A, Oishi H, Ema M, Takahashi S, Kishimoto H, Yanagisawa J
PloS one 2011;6(10):e25871
PloS one 2011;6(10):e25871
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE GTX114781 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: IMR32 B: JurKat 10% SDS PAGE GTX114781 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BAF57 antibody detects SMARCE1 protein at nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SMARCE1 protein stained by BAF57 antibody (GTX114781) diluted at 1:500.Blue: Hoechst 33343 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BAF57 antibody detects BAF57 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BAF57 protein stained by BAF57 antibody (GTX114781) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using BAF57(GTX114781) antibody at 1:500 dilution.
