PAB29379

antibody from Abnova Corporation

Targeting: ABHD12B BEM46L3, C14orf29

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29379

Provider

Abnova Corporation

Product name

ABHD12B polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human ABHD12B.

Antigen sequence

LGTGVATNAAKVLEEKGCPVDAIVLEAPFTNMWVA
SINYPLLKIYRNIPGFLRTLMDALRKDKIVFPNDE
NVKFLSSPLLILHGEDDRTVPLEYGKKLYEIARNA
YRNKERVKMVIFPPGFQHNLLCKSPTLLITVRDFL
SKQ


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with ABHD12B polyclonal antibody (Cat # PAB29379) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with ABHD12B polyclonal antibody (Cat # PAB29379) at 1-4 ug/mL concentration shows positivity in nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with ABHD12B polyclonal antibody (Cat # PAB29379) shows strong nuclear positivity in cells of seminiferus ducts at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)