26368-1-AP

antibody from Invitrogen Antibodies

Targeting: RNF14 ARA54, HFB30, TRIAD2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

26368-1-AP

Provider

Invitrogen Antibodies

Product name

RNF14 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MQFLKEETL AYLNIVSPFE LKIGSQKKVQ RRTAQASPNT ELDFGGAAGS DVDQEEIVDE RAVQDVESLS NLIQEILDFD QAQQIKCFNS KLFLCSICFC EKLGSECMYF LECRHVYCKA CLKDYFEIQI RDGQVQCLNC PEPKCPSVAT PGQVKELVEA ELFARYDRLL LQSSLDLMAD VVYCPRPCCQ LPVMQEPGCT MGICSSCNFA FCTLCRLTYH GVSPCKVTAE KLMDLRNEYL QADEANKRLL DQRYGKRVIQ KALEEMESKE WLEKNSKSCP CCGTPIEKLD GCNKMTCTGC MQYFCWICMG SLSRANPYKH FNDPGSPCFN RLFYAVDVDD DIWEDEVED (127-474 aa encoded by BC126185)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293T cells were subjected to SDS PAGE followed by western blot with 26368-1-AP ( RNF14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 26368-1-AP (RNF14 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HEK-293 cells using 26368-1-AP (RNF14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 26368-1-AP ( RNF14 Antibody) at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RNF14 (IP:26368-1-AP, 4ug; Detection:26368-1-AP 1:600) with COLO 320 cells lysate 4000ug.