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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21939 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Activin A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A549, H1299, HCT-116. Predicted reactivity: Mouse (100%), Rat (100%), Cat (100%), Pig (100%), Rabbit (100%), Chicken (100%), Sheep (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.66 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Senescence marker activin A is increased in human diabetic kidney disease: association with kidney function and potential implications for therapy.
Bian X, Griffin TP, Zhu X, Islam MN, Conley SM, Eirin A, Tang H, O'Shea PM, Palmer AK, McCoy RG, Herrmann SM, Mehta RA, Woollard JR, Rule AD, Kirkland JL, Tchkonia T, Textor SC, Griffin MD, Lerman LO, Hickson LJ
BMJ open diabetes research & care 2019;7(1):e000720
BMJ open diabetes research & care 2019;7(1):e000720
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Inhibin beta-A using 30 µg of A) H1299 (B) HeLa S3 and C) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Inhibin beta-A polyclonal antibody (Product # PA5-21939) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Activin A was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Activin A Polyclonal Antibody (Product # PA5-21939) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Activin A Polyclonal Antibody (Product # PA5-21939). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Activin A Polyclonal Antibody (Product # PA5-21939) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Inhibin beta-A in paraformaldehyde-fixed HeLa cells using an Inhibin beta-A polyclonal antibody (Product # PA5-21939) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Inhibin beta-A (Product # PA5-21939) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CLEAR CELL OVCA xenograft, using Inhibin beta A (Product # PA5-21939) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 In vitro models of DKD-related injury. HK-2 studies following incubation with high glucose (HG, 25 mmol/L), transforming growth factor-beta1 (TGF-beta1; 5 ng/mL) and albumin (ALB, 5 mg/mL). Time-dependent effect of HG+TGF-beta1 on the mRNA/gene expression of activin A, monocyte chemoattractant protein-1 (MCP-1; an inflammatory marker), type I collagen (mesenchymal marker) and E-cadherin (epithelial marker) in injured HK-2 cells (A) Immunofluorescent staining of HK-2 cells injured by HG+TGF-beta1 at 12 hours (h) compared with baseline (B) Time-dependent effect for activin A and p16 expression in injured HK-2 cells (C) Protein levels of activin A in the supernatant of HK-2 treated with HG+TGF-beta1 (D) and combinations of HG with or without ALB (E) at 24 hours. Values are mean+-SEM. a P
