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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00008539-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008539-M01, RRID:AB_2058228
- Product name
- Mouse Monoclonal API5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. API5 (1C2)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Api5 contributes to E2F1 control of the G1/S cell cycle phase transition.
Garcia-Jove Navarro M, Basset C, Arcondéguy T, Touriol C, Perez G, Prats H, Lacazette E
PloS one 2013;8(8):e71443
PloS one 2013;8(8):e71443
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: API5 Antibody (1C2) [H00008539-M01] - API5 monoclonal antibody (M01), clone 1C2 Analysis of API5 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: API5 Antibody (1C2) [H00008539-M01] - Detection limit for recombinant GST tagged API5 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: API5 Antibody (1C2) [H00008539-M01] - Analysis of monoclonal antibody to API5 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: API5 Antibody (1C2) [H00008539-M01] - Analysis of monoclonal antibody to API5 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
