25689-1-AP

antibody from Invitrogen Antibodies

Targeting: API5 AAC-11, AAC11, API5L1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

25689-1-AP

Provider

Invitrogen Antibodies

Product name

API5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LSTKLKTLP DEVLTKEVEE LILTESKKVL EDVTGEEFVL FMKILSGLKS LQTVSGRQQL VELVAEQADL EQTFNPSDPD CVDRLLQCTR QAVPLFSKNV HSTRFVTYFC EQVLPNLGTL TTPVEGLDIQ LEVLKLLAEM SSFCGDMEKL ETNLRKLFDK LLEYMPLPPE EAENGENAGN EEPKLQFSYV ECLLYSFHQL GRKLPDFLTA KLNAEKLKDF KIRLQYFARG LQVYIRQLRL ALQGKTGEAL KTEENKIKVV ALKITNNINV LIKDLFHIPP SYKSTVTLSW KPVQKVEIGQ KRASEDTTSG SPPKKSSAGP KRDARQIYNP PSGKYSSNLG NFNYERSLQG K (155-504 aa encoded by BC017709)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.22 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP ( API5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 25689-1-AP (API5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 25689-1-AP (API5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 25689-1-AP ( API5 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 25689-1-AP ( API5 Antibody) at dilution of 1:50 (under 40x lens).