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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00010146-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010146-M01, RRID:AB_539018
- Product name
- Mouse Monoclonal G3BP1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. G3BP - Ras-GTPase-activating protein SH3-domain-binding protein
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Mammalian orthoreovirus Infection is Enhanced in Cells Pre-Treated with Sodium Arsenite.
G3BP1 promotes DNA binding and activation of cGAS.
Preferential binding of a stable G3BP ribonucleoprotein complex to intron-retaining transcripts in mouse brain and modulation of their expression in the cerebellum.
5-Fluorouracil affects assembly of stress granules based on RNA incorporation.
Deficiency of G3BP1, the stress granules assembly factor, results in abnormal synaptic plasticity and calcium homeostasis in neurons.
Progressive loss of dopaminergic neurons induced by unilateral rotenone infusion into the medial forebrain bundle.
Lutz MM 4th, Worth MP, Hinchman MM, Parker JSL, Ledgerwood ED
Viruses 2019 Jun 18;11(6)
Viruses 2019 Jun 18;11(6)
G3BP1 promotes DNA binding and activation of cGAS.
Liu ZS, Cai H, Xue W, Wang M, Xia T, Li WJ, Xing JQ, Zhao M, Huang YJ, Chen S, Wu SM, Wang X, Liu X, Pang X, Zhang ZY, Li T, Dai J, Dong F, Xia Q, Li AL, Zhou T, Liu ZG, Zhang XM, Li T
Nature immunology 2019 Jan;20(1):18-28
Nature immunology 2019 Jan;20(1):18-28
Preferential binding of a stable G3BP ribonucleoprotein complex to intron-retaining transcripts in mouse brain and modulation of their expression in the cerebellum.
Martin S, Bellora N, González-Vallinas J, Irimia M, Chebli K, de Toledo M, Raabe M, Eyras E, Urlaub H, Blencowe BJ, Tazi J
Journal of neurochemistry 2016 Nov;139(3):349-368
Journal of neurochemistry 2016 Nov;139(3):349-368
5-Fluorouracil affects assembly of stress granules based on RNA incorporation.
Kaehler C, Isensee J, Hucho T, Lehrach H, Krobitsch S
Nucleic acids research 2014 Jun;42(10):6436-47
Nucleic acids research 2014 Jun;42(10):6436-47
Deficiency of G3BP1, the stress granules assembly factor, results in abnormal synaptic plasticity and calcium homeostasis in neurons.
Martin S, Zekri L, Metz A, Maurice T, Chebli K, Vignes M, Tazi J
Journal of neurochemistry 2013 Apr;125(2):175-84
Journal of neurochemistry 2013 Apr;125(2):175-84
Progressive loss of dopaminergic neurons induced by unilateral rotenone infusion into the medial forebrain bundle.
Norazit A, Meedeniya AC, Nguyen MN, Mackay-Sim A
Brain research 2010 Nov 11;1360:119-29
Brain research 2010 Nov 11;1360:119-29
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of G3BP over-expressed 293 cell line, cotransfected with G3BP Validated Chimera RNAi ( Cat # H00010146-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with G3BP monoclonal antibody (M01), clone 2F3 (Cat # H00010146-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of G3BP expression in transfected 293T cell line by G3BP monoclonal antibody (M01), clone 2F3.Lane 1: G3BP transfected lysate(52.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - G3BP monoclonal antibody (M01), clone 2F3 Analysis of G3BP expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: G3BP1 Antibody (2F3) [H00010146-M01] - Detection limit for recombinant GST tagged G3BP1 is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of monoclonal antibody to G3BP on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of monoclonal antibody to G3BP on formalin-fixed paraffin-embedded human lymphoma. Antibody concentration 1 ug/ml.
