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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- NBP1-18922 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-18922, RRID:AB_1625710
- Product name
- Rabbit Polyclonal G3BP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Mammalian Orthoreovirus Factories Modulate Stress Granule Protein Localization by Interaction with G3BP1.
Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules.
Choudhury P, Bussiere LD, Miller CL
Journal of virology 2017 Nov 1;91(21)
Journal of virology 2017 Nov 1;91(21)
Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules.
Fujimura K, Sasaki AT, Anderson P
Nucleic acids research 2012 Sep;40(16):8099-110
Nucleic acids research 2012 Sep;40(16):8099-110
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. Affinity purified rabbit anti-G3BP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. G3BP1 was also immunoprecipitated by rabbit anti-G3BP1 antibody NBP1-18923, which recognizes a downstream epitope.
