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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [5]
- Immunohistochemistry [4]
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- Product number
- MA5-31363 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TUFM Monoclonal Antibody (CL2242)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AVDTYIPVPA RDLEKPFLLP VEAVYSVPGR GTVVTGTLER GILKKGDECE LLGHSKNIRT VVTGIEMFHK SLERAEAGDN LGALVRGLKR EDLRRGLVMV KPGSI
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL2242
- Vial size
- 100 µL
- Concentration
- 1.00 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Inhibition of mitochondrial translation suppresses glioblastoma stem cell growth.
Sighel D, Notarangelo M, Aibara S, Re A, Ricci G, Guida M, Soldano A, Adami V, Ambrosini C, Broso F, Rosatti EF, Longhi S, Buccarelli M, D'Alessandris QG, Giannetti S, Pacioni S, Ricci-Vitiani L, Rorbach J, Pallini R, Roulland S, Amunts A, Mancini I, Modelska A, Quattrone A
Cell reports 2021 Apr 27;35(4):109024
Cell reports 2021 Apr 27;35(4):109024
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TUFM by a TUFM monoclonal antibody (Product # MA5-31363). Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TUFM by a TUFM monoclonal antibody (Product # MA5-31363). Lane 1: Marker [kDa] Lane 2: Human cell line U-251 MG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TUFM by a TUFM monoclonal antibody (Product # MA5-31363). Lane 1: Marker [kDa] Lane 2: Human cell line HeLa cytoplasmic fraction Lane 3: Human cell line HeLa membrane fraction Lane 4: Human cell line HeLa nuclear fraction Lane 5: Human cell line HeLa chromatin fraction Lane 6: Human cell line HeLa cytoskeletal fraction.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TUFM in HeLa cells using a TUFM monoclonal antibody (Product # MA5-31363). The analysis shows distinct mitochondrial staining in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TUFM in A431 cells using a TUFM monoclonal antibody (Product # MA5-31363). The analysis shows distinct mitochondrial staining in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TUFM in MCF7 cells using a TUFM monoclonal antibody (Product # MA5-31363). The analysis shows distinct mitochondrial staining in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TUFM in U2OS cells using a TUFM monoclonal antibody (Product # MA5-31363). The analysis shows distinct mitochondrial staining in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TUFM in U251 cells using a TUFM monoclonal antibody (Product # MA5-31363). The analysis shows distinct mitochondrial staining in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TUFM in human fallopian tube using a TUFM monoclonal antibody (Product # MA5-31363). The analysis shows strong granular cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TUFM in human duodenum using a TUFM monoclonal antibody (Product # MA5-31363). The analysis shows strong granular cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TUFM in human kidney using a TUFM monoclonal antibody (Product # MA5-31363). The analysis shows strong granular cytoplasmic positivity in cells in tubules and moderate staining in glomerular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TUFM in human testis using a TUFM monoclonal antibody (Product # MA5-31363). The analysis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and in Leydig cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Genetic inhibition of mitochondrial translation suppresses GSC growth, recapitulating Q/D effects (A) Competition assay in VIPI cells transduced with viruses expressing sgRNAs for target genes. Data were normalized to day 1. n = 4 biological replicates, mean +- SD. (B) Percentage of insertions or deletions (indels) analyzed by decomposition (tracking of indels by decomposition [TIDE]) analysis in Cas9-expressing COMI and VIPI cells following lentiviral transduction of the sgRNAs selected in (A) (sgTUFM_1 and sgMRPS18A_1). n = 3-4 biological replicates, mean +- SD. (C) Effects of TUFM and MRPS18A knockout on TUFM, MRPS18A, and beta-tubulin proteins in COMI and VIPI cells, as assayed by immunoblotting. One representative result is shown; n = 2 biological replicates. (D) Effects of TUFM and MRPS18A knockout on COX1, COX4, and beta-tubulin proteins in COMI and VIPI cells, as assayed by immunoblotting. One representative result is shown; n = 2 biological replicates. (E) Effects of TUFM and MRPS18A knockout on COMI and VIPI cells grown in suspension. Shown are example images from days 0, 4, 10, and 15; scale bar, 200 mum. (F) Sphere area measured over the course of the 15-day experiment. The data were normalized to day 0. n = 15 technical replicates, mean +- SEM. One representative experiment is shown; n = 3 biological replicates. (G) Effects of TUFM and MRPS18A knockout on COMI and VIPI proliferation when grown as adherent cultures. The data were normalized to day 0. n =
