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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 25576-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C2orf79/PTRHD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MHRGVGPAF RVVRKMAASG AEPQVLVQYL VLRKDLSQAP FSWPAGALVA QACHAATAAL HTHRDHPHTA AYLQELGRMR KVVLEAPDET TLKELAETLQ QKNIDHMLWL EQPENIATCI ALRPYPKEEV GQYLKKFRLF K (1-140 aa encoded by BC073803)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 25576-1-AP ( C2orf79/PTRHD1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using 25576-1-AP (C2orf79/PTRHD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 25576-1-AP ( C2orf79/PTRHD1 antibody at dilution of 1:600 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 25576-1-AP ( C2orf79/PTRHD1 antibody at dilution of 1:600 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-C2orf79/PTRHD1 (IP:25576-1-AP, 4ug; Detection:25576-1-AP 1:500) with COLO 320 cells lysate 4000ug.
