PA5-30175

antibody from Invitrogen Antibodies

Targeting: SMARCD1 BAF60A, CRACD1, Rsc6p

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30175

Provider

Invitrogen Antibodies

Product name

SMARCD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, HeLa nuclear extract, NIH-3T3.

Concentration

0.84 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SMARCD1 using 30 µg of A549 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SMARCD1 polyclonal antibody (Product # PA5-30175) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SMARCD1 was performed by separating 30 µg of HeLa whole cell and nuclear extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a SMARCD1 Polyclonal Antibody (Product # PA5-30175) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SMARCD1 Polyclonal Antibody (Product # PA5-30175). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 10% SDS PAGE. SMARCD1 Polyclonal Antibody (Product # PA5-30175) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SMARCD1 in paraformaldehyde-fixed HeLa cells using a SMARCD1 polyclonal antibody (Product # PA5-30175) at a 1:200 dilution.