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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-17385 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-NFkB p52 (Ser866, Ser870) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 64 µg/mL
- Storage
- -20°C
Submitted references Lipoxin A4 promotes autophagy and inhibits overactivation of macrophage inflammasome activity induced by Pg LPS.
The role of NFκB in spheroid formation of human breast cancer cells cultured on the Random Positioning Machine.
Zhao J, Geng W, Wan K, Guo K, Xi F, Xu X, Xiong X, Huang X, Liu J, Kuang X
The Journal of international medical research 2021 Feb;49(2):300060520981259
The Journal of international medical research 2021 Feb;49(2):300060520981259
The role of NFκB in spheroid formation of human breast cancer cells cultured on the Random Positioning Machine.
Kopp S, Sahana J, Islam T, Petersen AG, Bauer J, Corydon TJ, Schulz H, Saar K, Huebner N, Slumstrup L, Riwaldt S, Wehland M, Infanger M, Luetzenberg R, Grimm D
Scientific reports 2018 Jan 17;8(1):921
Scientific reports 2018 Jan 17;8(1):921
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-NF-kappa B2 p100 pSer866/870 in extracts from HeLa cells transfected with wild-type or mutant NF-kappaB2 p100 (SS866/870AA) using Phospho-NF-kappa B2 p100 pSer866/870 polyclonal antibody (Product # PA5-17385).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 ( A ) NFKB1 gene expression; ( B ) NFkBp50 Western blot analysis; ( C ) phosphorylated (p)-NFkappaBp50 Western blot analysis; ( D ) NFKB2 gene expression; ( E ) p-NFkappaBp52 Western blot analysis; ( F ) NFKB3 gene expression; ( G ) NFkappaBp65 Western blot analysis; ( H ) p-NFkBp65 Western blot analysis; ( I ) NFKBIA gene expression; ( J ) IkappaBalpha Western blot analysis; ( K ) p- IkappaBbeta Western blot analysis; ( L ) NFKBIB gene expression; ( M ) NFKBIE gene expression; ( N ) IKBKG gene expression. The position (arrow) and molecular size (in kD) of the investigated proteins are indicated on each of the Western blot membrane images. Cofilin 1 was used as loading control. The vertical lines indicate group separation giving n = 5 per group.
