14333-1-AP

antibody from Invitrogen Antibodies

Targeting: KIF3C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14333-1-AP

Provider

Invitrogen Antibodies

Product name

KIF3C Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MASKTKASE ALKVVARCRP LSRKEEAAGH EQILTMDVKL GQVTLRNPRA APGELPKTFT FDAVYDASSK QADLIDSVLQ GFNGTVFAYG QTGTGKTYTM QGTWVEPELR GVIPNAFEHI FTHISRSQNQ QYLVRASYLE IYQEEIRDLL SKEPGKRLEL KENPETGVYI KDLSSFVTKN VKEIEHVMNL GNQTRAVGST HMNEVSSRSH AIFIITVECS ERGSDGQDHI RVGKLNLVDL AGSERQNKAG PNTAGGAATP SSGGGGGGGG SGGGAGGERP KEASKINLSL SALGNVIAAL AGNRSTHIPY RDSKLTRLLQ DSLGGNAKTI MVATLGPASH SYDESLSTLR FANRAKNIKN K (1-360 aa encoded by BC042486)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14333-1-AP (KIF3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14333-1-AP (KIF3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 14333-1-AP (KIF3C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using (Product # 14333-1-AP) (KIF3C antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14333-1-AP ( KIF3C antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14333-1-AP ( KIF3C antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-KIF3C (IP:14333-1-AP, 4ug; Detection:14333-1-AP 1:500) with mouse brain tissue lysate 3440ug.