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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP2-32093 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal DNA polymerase delta p50 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA polymerase delta p50 Antibody [NBP2-32093] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PolD2 antibody NBP2-32093 used for IP at 6 ug per reaction. PolD2 was also immunoprecipitated by rabbit anti-PolD2 antibody BL15767. For blotting immunoprecipitated PolD2, NBP2-32093was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA polymerase delta p50 Antibody [NBP2-32093] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PolD2 antibody NBP2-32093 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: DNA polymerase delta p50 Antibody [NBP2-32093] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PolD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
