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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00003172-M04 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003172-M04, RRID:AB_565840
- Product name
- HNF4A monoclonal antibody (M04), clone 4E2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant HNF4A.
- Antigen sequence
NDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFI
KLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHL
MQEHMGTNVIVANTMPTHLSNGQMCEWPRP- Isotype
- IgG
- Antibody clone number
- 4E2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HNF4A monoclonal antibody (M04), clone 4E2 Western Blot analysis of HNF4A expression in HepG2 ( Cat # L019V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HNF4A monoclonal antibody (M04), clone 4E2. Western Blot analysis of HNF4A expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody (M04), clone 4E2.Lane 1: HNF4A transfected lysate(51.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged HNF4A is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
