PAB21164

antibody from Abnova Corporation

Targeting: POLDIP3 KIAA1649, PDIP3, PDIP46, SKAR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21164

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21164, RRID:AB_10970592

Product name

POLDIP3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant POLDIP3.

Antigen sequence

SLDELIRKRGAAAKGRLNARPGVGGVRSRVGIQQG
LLSQSTRTATFQQRFDARQKIGLSDARLKLGVKDA
REKLLQKDARFRIKGKVQDAR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with POLDIP3 polyclonal antibody (Cat # PAB21164) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with POLDIP3 polyclonal antibody (Cat # PAB21164) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with POLDIP3 polyclonal antibody (Cat # PAB21164) shows strong nuclear positivity in cells in seminiferus ducts at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)